Wikinventia — Atlas of discoveries and inventions · Industrial Age

Fluorescence microscopy — Köhler and Siedentopf

1911 AD · Transmission: Global
OpticsMethodGermanic

In 1911 Otto Heimstädt published the description of the first practical fluorescence microscope, developed in the Zeiss environment. The principle differs from bright-field microscopy: instead of observing transmitted or reflected light, the sample is illuminated with high-energy light and the longer-wavelength fluorescent emission is detected. This change in contrast transformed microscopy: instead of seeing shapes, it became possible to make specific molecules, selected structures, or dynamic processes visible. Modern fluorescence microscopy, combined with genetic markers such as GFP (Nobel 2008), is the basis of contemporary cellular and molecular biological imaging.

InstitutionCarl Zeiss Jena
Historical regionJena, Germany
Primary sourceHeimstädt, O. — "Das Fluoreszenzmikroskop" (Zeitschrift für wissenschaftliche Mikroskopie, 1911)
Secondary sourceFSU Optics Timeline — https://osa.magnet.fsu.edu/opticstimeline/
Original languageGerman
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